Abstract
Purpose:To develop a suitable ratio of n-6 to n-3 polyunsaturated fatty acid (PUFAs) emulsions for intravenous injection containing sea buckthorn seed oil (SBSO). Methods:The content of fatty acids (FAs) in the SBSO for injection was determined using high-performance liquid chromatography (HPLC) methods. Sea buckthorn seed oil fat emulsion (SBFE) was prepared by high-shear mixing and high pressure homogenization and sterilized in a 121˚C rotating water bath for 15 min. Its physicochemical stability was evaluated by examining its appearance and determining the pH value, particle size, ζ-potential and peroxide value. Result:The proportion of FAs in the SBSO for injection were 30%–45% linoleic acid (LA, 18:2 n-6), 20%–32% α-linolenic acid (ALA,18:3n-3), 16%–28% oleic acid (18:1, n-9), 5%–12% palmitic acid (16:0), and 0.5%–3% stearic acid (18:0). The ratio of n-3 PUFAs to n-6 PUFAs in SBSO was 1:1–1:2. The formulation of SBFE was composed of SBSO for injection 10% (w/v), soya lecithin 1.2% (w/v), Pluronic F68 0.2% (w/v), Tween 80 0.2% (w/v), glycerin 2.5% (w/v), EDTA 0.005% (w/v) and Na2SO3 0.025% (w/v) with a pH of 7.5–8.5. The SBFE had a particle size of 174.4±59.1 nm, and a ζ-potential of-25.5mv while the peroxide value was zero; it remained stable for 6 months at 4±2˚C and 25±2˚C. Conclusions:Overall, the SBFE described in this paper is a new nutritional fat emulsion, which is suitable for industrial-scale production and clinical application.
Keywords:
Sea buckthorn
Formulation
Intravenous
Fat emulsion
PUFAs
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